Protocol: RCA on Fresh Frozen Tissue¶
This page is retired
An early condensed fresh-frozen protocol (last at v5.3), superseded by the pre-treatment / library-construction split the rest of the site uses:
- Sectioning, fixation, permeabilization, dehydration → Sample Pre-treatment: Fresh Frozen
- Hybridization, ligation, RCA, post-fix → RCA Protocol
It had fallen out of the site navigation and its values had gone stale — it still carried the 2 h / high-concentration hybridization and reagent lists without stock or volume columns. Kept only so old links resolve.
(Its 10%-formamide stringent wash was listed here as stale when this page was retired in 2026-07, during the four weeks the default stood at 20%. RCA v7.0 returned the default to 10%, so that value was never actually wrong — the note is corrected rather than the history rewritten.)
Fresh frozen workflow: Pre-treatment (FF) → RCA Protocol → signal readout.
Changelog¶
- retired -- 2026-07-30 -- Superseded by pretreatment-ff.md + rca.md. See git history for the original text.